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Kernel Metadata Declaration — on demand

ISO 26324 asks a DOI Registration Agency to produce a Kernel Metadata Declaration for every DOI it issues. Give any DOI: its public record becomes a record in the Smart Scholars DOI Metadata Format 1.0, checked against every rule, and declared as the Kernel XML.

Reads the DOI's public Crossref record; nothing is stored. Until Smart Scholars is accredited, every Declaration is marked as a draft in the XML itself.

✓ Meets every rule of the format

The record below is complete and every controlled value is a DOI Attribute Value Set 2.3 spelling. The Declaration on the right follows DOIMetadataKernel.xsd element by element.

What the DOI identifies

Inhibition of Proliferation of K-562 Human Blood Cancer Cell Due To Opuntia Elatior Fruit Extract

10.46243/jst.2021.v06.i04.pp32-38 · JournalArticle — an article in a journal · Digital · Visual · Language · en

Published 2021-07-14

Part of Journal of Science & Technology · ISSN 2456-5660 · vol. 06 · no. 04 · pp. 32–38

Principal agents

  • Chirag U. Narayankar (author → Author)
  • M. Mane (author → Author)
  • R. Patil (author → Author)
  • Longman Publishers (publisher → Publisher)

Also in the record, outside the Kernel: the abstract, the licence, 4 links, 30 references. Source: Crossref (member 25296), registered 2026-09-08, last deposited 2026-09-20.

⬇ Record (JSON) ⬇ Declaration (XML)

System metadata — ISO 26324:2025, Annex B · DOI Handbook 10.1

Each element by the standard's name and the Handbook's (in grey), read off the record.

DOI Name
DOI name
10.46243/jst.2021.v06.i04.pp32-38
Referent Type
referentType
Creation
Referent Sub-Type
referentSubType
JournalArticle — an article in a journal
Referent Name(s)
referentName(s)
Inhibition of Proliferation of K-562 Human Blood Cancer Cell Due To Opuntia Elatior Fruit Extract (PrincipalTitle, en)
Basic Metadata
basicMetadata
author: Chirag U. Narayankar
author: M. Mane
author: R. Patil
publisher: Longman Publishers
published: 2021-07-14
part of: Journal of Science & Technology · ISSN 2456-5660 · vol. 06 · no. 04 · pp. 32–38
language: en
form: Digital · Visual · Language
Referent Identifier(s)
alternateIdentifier(s)
none besides the DOI
Registration Authority
registrationAuthorityCode
Crossref — issued by Crossref (member 25296); held here as a copy
Created Date
issueDate
2026-09-08
relatedIdentifiersnone needed — the descriptive metadata is in this record

The record

{
    "format": "smartscholars-doi-metadata/1.0",
    "doi": "10.46243/jst.2021.v06.i04.pp32-38",
    "referent": "Creation",
    "type": "JournalArticle",
    "structural_type": "Digital",
    "modes": [
        "Visual"
    ],
    "characters": [
        "Language"
    ],
    "titles": [
        {
            "value": "Inhibition of Proliferation of K-562 Human Blood Cancer Cell Due To Opuntia Elatior Fruit Extract",
            "type": "PrincipalTitle",
            "lang": "en"
        }
    ],
    "identifiers": [
        {
            "type": "DOI",
            "value": "10.46243/jst.2021.v06.i04.pp32-38"
        }
    ],
    "agents": [
        {
            "role": "author",
            "name": {
                "given": "Chirag U.",
                "family": "Narayankar"
            },
            "sequence": "first"
        },
        {
            "role": "author",
            "name": {
                "given": "",
                "family": "M. Mane"
            },
            "sequence": "additional"
        },
        {
            "role": "author",
            "name": {
                "given": "",
                "family": "R. Patil"
            },
            "sequence": "additional"
        },
        {
            "role": "publisher",
            "name": {
                "org": "Longman Publishers"
            }
        }
    ],
    "dates": {
        "published": "2021-07-14",
        "date_type": "PublicationDate",
        "online": "2021-07-14"
    },
    "language": "en",
    "container": {
        "type": "Journal",
        "titles": [
            {
                "value": "Journal of Science & Technology",
                "type": "PrincipalTitle"
            }
        ],
        "identifiers": [
            {
                "type": "ISSN",
                "value": "2456-5660",
                "medium": "electronic"
            }
        ],
        "volume": "06",
        "issue": "04",
        "pages": {
            "first": "32",
            "last": "38"
        }
    },
    "links": [
        {
            "url": "https://www.jst.org.in/index.php/pub/article/view/433",
            "return_type": "text/html",
            "primary": true
        },
        {
            "url": "https://www.jst.org.in/index.php/pub/article/download/433/385",
            "purpose": "text-mining",
            "return_type": "application/pdf"
        },
        {
            "url": "https://www.jst.org.in/index.php/pub/article/download/433/2921",
            "purpose": "text-mining",
            "return_type": "application/xml"
        },
        {
            "url": "https://www.jst.org.in/index.php/pub/article/view/433/385",
            "purpose": "similarity-checking"
        }
    ],
    "abstract": {
        "value": "The ethanolic extract of Opuntia elatior was studied for anticancer potential against human blood cancer cell line K-562. The cytotoxicity of ethanolic extract was analyzed, using MTT assay and flow cytometric analysis. It was noticed that IC50 value of Opunria elatior curde extract was 74.32 µl/ml showing its cytotoxicity in the human blood cancer cell line by using MTT assay. The flow cytometry evaluation exhibits cell arrested in G2/M and S phases. Cell arrested due to Opuntia elatior extract shows higher than positive control Cisplatin. Thus Opuntia elatior fruit extract might be used as potent source of anticancerous compound against human blood cancer",
        "lang": "en"
    },
    "license": {
        "url": "https://creativecommons.org/licenses/by/4.0/",
        "start": "2021-07-14",
        "applies_to": "vor"
    },
    "references": [
        {
            "key": "ref1",
            "unstructured": "Aziz, A., Taha H., Mohebali N., Chung Y. L., Ismail N. H., Bakar M. Z. A. and Yusof F. Z. M., 2016. Anti-Cancer Potential of Pseuduvaria Macrophylla in Human Cancer Cell lines. Journal of Advanced Research in Applied Sciences and Engineering Technology. 4 (1), 1- 11"
        },
        {
            "key": "ref2",
            "doi": "10.1107/s2053229617018551",
            "unstructured": "Bhat S. S., Revankar V. K., Kumbar V., Bhat K. and Kawade V. A., 2018. Synthesis, crystal structure and biological properties of a cisdichloridobis (diimine)copper(II) complex. Acta Cryst. 74, 1-6"
        },
        {
            "key": "ref3",
            "doi": "10.1007/s12033-010-9321-2",
            "unstructured": "Baguley B.C., 2010. Multiple drug resistance mechanisms in cancer. Mol. Biotechnol. 46, 308- 316"
        },
        {
            "key": "ref4",
            "doi": "10.1002/jcp.22963",
            "unstructured": "Chen Y. J. and L. S. Chang., 2011. Arecoline-Induced Death of Human Leukemia K562 Cells is Associated with Surface Up-Modulation of TNFR2. Journal of Cellular Physiology. 227, 2240–2251"
        },
        {
            "key": "ref5",
            "doi": "10.3390/molecules190914879",
            "unstructured": "El-Mostafa K., El-Kharrassi Y., Badreddine A., Andreoletti P., Vamecq J., El-Kebbaj M.S., Latruffe N., Lizard G., Nasser B., Cherkaoui-Malki M., 2014. Nopal cactus (Opuntia ficus-indica) as a source of bioactive compounds for nutrition, health and disease. Molecules. 19 (4), 14879-91"
        },
        {
            "key": "ref6",
            "doi": "10.4149/bll_2016_126",
            "unstructured": "Ertekin T., Bozkurt O., Eroz R., Nisari M., Bircan D., Nisari M., Unur E., 2016. May argyrophilic nucleolar organizing region-associated protein synthesis be used for selecting the most reliable dose of drugs such as rhamnetin in cancer treatments?. Bratisl Med J. 117 (11), 653–658"
        },
        {
            "key": "ref7",
            "doi": "10.1016/j.imr.2017.01.004",
            "unstructured": "Ghagane S. C., Puranik S. I., Kumbhar V. M., Nerli R. B., Jalapure S. S. Hiremath M.B.,Neelagund S., Aladakatti R., 2017. In vitro antioxidant and anticancer activity of Leea indica leaf extract oh human prostate cancer cell lines. Integrative Medicines Research. 6 (1), 79-87"
        },
        {
            "key": "ref8",
            "doi": "10.1039/c5sc03600c",
            "unstructured": "Gong Q., Shi W., Li L. and Ma H., 2015. Leucine Aminopeptidase May Contribute to Intrinsic Resistance of Cancer Cells toward Cisplatin as Revealed by an Ultrasensitive Fluorescent Probe. Chemical Science. 00, 1-5"
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        {
            "key": "ref9",
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            "unstructured": "Gupta R. S., Murray W. and Gupta R., 1988. Cross resistance pattern towards anticancer drugs of a human carcinoma multidrug-resistant cell line. Br. J. Cancer. 58, 441-447"
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        {
            "key": "ref10",
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            "unstructured": "Hail J. N., 2005. Mitochondria: a novel target for the chemoprevention of cancer. Apoptosis. 10, 687–705"
        },
        {
            "key": "ref11",
            "unstructured": "Hsu H.J., Chang C.P., Yang Y.H., Shieh T.Y., 2001. The effect of arecoline on the release of cytokines using cultured peripheral blood mononuclear cell from patients with oral mucous diseases. Kaohsiung J Med Sci. 17, 175-182"
        },
        {
            "key": "ref12",
            "doi": "10.1093/carcin/bgg083",
            "unstructured": "Jeng J.H., Wang Y.J., Chiang B.L., Lee P.H., Chan C.P., Ho Y.S., Wang T.M., Lee J.J., Hahn L.J., Chang M.C., 2003. Roles of keratinocyte inflammation in oral cancer: Regulating the prostaglandin E2, interleukin-6 and TNF-α production of oral epithelial cells by areca nut extract and arecoline. Carcinogenesis. 24, 1301-1315"
        },
        {
            "key": "ref13",
            "doi": "10.1002/ijc.20418",
            "unstructured": "Keshavan P., Schwemberger S. J., Smith D. L.H., Babcock G. F. and Zucker S. D., 2004. Unconjugated Bilirubin Induces Apoptosis In Colon Cancer Cells By Triggering Mitochondrial Depolarization. Int. J. Cancer. 112, 433–445"
        },
        {
            "key": "ref14",
            "doi": "10.1002/1097-0142(197907)44:1<58::aid-cncr2820440111>3.0.co;2-y",
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        },
        {
            "key": "ref15",
            "doi": "10.3892/ol.2018.9575",
            "unstructured": "Lan L., Wang Y., Pan Z., Wang B., Yue Z., Jiang Z., Li L., Wang C. and Tang H., 2018. Rhamnetin induces apoptosis in human breast cancer cells via the miR‑34a/Notch‑1 signaling pathway. Oncology Letters. 17, 676-682"
        },
        {
            "key": "ref16",
            "doi": "10.1158/0008-5472.can-07-2235",
            "unstructured": "Lee M. S., Johansen L., Zhang Y., Wilson A., Keegan M., Avery W., Elliott P., Borisy A. A. and Keith C. T., 2007. The Novel Combination of Chlorpromazine and Pentamidine Exerts Synergistic Antiproliferative Effects through Dual Mitotic Action. Cancer Res. 67 (23), 11359- 11367"
        },
        {
            "key": "ref17",
            "doi": "10.1002/tox.22204",
            "unstructured": "Lin Y. M., Kuo W. W, Velmurugan B. K., Hsien H. H., Hsieh Y.L., Hsu H.H., Tu C.C., Bau D.T., Viswanadha V. P., Huang C.Y., 2015. Helioxanthin suppresses the cross talk of COX- 2/PGE2 and EGFR/ERK pathway to inhibit Arecoline-induced Oral Cancer Cell (T28) proliferation and blocks tumor growth in xenografted nude mice. Environmental Toxicology. 31, 2045-2056"
        },
        {
            "key": "ref18",
            "unstructured": "Mehrzad J. and Rajabi M., 2011. Kinetin (N6-furfuryladenine): Cytotoxicity against MCF-7 breast cancer cell line and interaction with bovine serum albumin. African Journal of Biotechnology. 10 (33), 6304-6309"
        },
        {
            "key": "ref19",
            "unstructured": "Moammadpour R., Safarian S., Farahnak S., Hasheminasl S., Sheibani N., 2012. Journal of Cell and Molecular Biology. 10 (1), 41-54"
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        {
            "key": "ref20",
            "doi": "10.1385/1-59259-811-0:301",
            "unstructured": "Pozarowski P.and Darzynkiewicz Z., 2004. Analysis of cell cycle by flow cytometry. Methods Mol Biol.281, 301-11"
        },
        {
            "key": "ref21",
            "doi": "10.1016/j.bbrc.2006.02.074",
            "unstructured": "Rao P., Suzuki R., Mizobuchi S., Yamaguchi T., Sasaguri S., 2006. Bilirubin exhibits a novel anti- cancer effect on human adenocarcinoma. Biochemical and Biophysical Research Communication. 342, 1279-1283"
        },
        {
            "key": "ref22",
            "doi": "10.1007/s00280-006-0216-7",
            "unstructured": "Rickardson L., Fryknas M., Lovborg C. H. H., Nygren P., Gustafsson M. G., Isaksson A., Larsson R., 2006. Cancer Chemother Pharmacol. 58, 749–758"
        },
        {
            "key": "ref23",
            "doi": "10.1038/sj.onc.1206933",
            "unstructured": "Siddik Z. H., 2003. Cisplatin: mode of cytotoxic action and molecular basis of resistance Oncogene. 22, 7265-7279"
        },
        {
            "key": "ref24",
            "doi": "10.3109/08923979309026000",
            "unstructured": "Selvan R.S. and Rao A.R. 1993. Influence of arecoline on immune system: III. Suprression of B cell- mediated immune response in mice after shorter-term exposure. Immunophermacol Immunotoxicol. 15, 291-305"
        },
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            "doi": "10.1093/carcin/bgp263",
            "unstructured": "Thun M. J., Lancey J. O., Center M. M. and Ward E. M. 2009. The global burden of cancer: priorities for prevention. Carcinogenesis. 31 (1), 100-110.2"
        },
        {
            "key": "ref26",
            "unstructured": "Tobey R. A. and Ley K. D., 1971. Isoleucine-mediated Regulation of Genome Replication in Various Mammalian Cell Lines. Cancer Research. 31, 46-51"
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        {
            "key": "ref27",
            "doi": "10.1016/j.tox.2008.05.007",
            "unstructured": "Tsai Y.S., Lee K.W., Huang J.L., Liu Y.S., Juo S.H.H. Kuo W.R., Chang J.G., Lin C.S., Jong Y.S., 2008. Arecoline, a major alkaloid of areca nut, inhibits p53, respresses DNA repair, and triggers DNA damage response in human epithellial cells. Toxicol. 249, 230-237"
        },
        {
            "key": "ref28",
            "doi": "10.1111/j.1349-7006.2008.00989.x",
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        {
            "key": "ref29",
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            "unstructured": "Xiao F., Wang C., Yin H., Yu J., Chen S., Fang J., Guo F., 2016. Leucine deprivation inhibits proliferation and induces apoptosis of human breast cancer cells via fatty acid synthase. Oncotarget. 7 (39), 63679- 63689"
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        {
            "key": "ref30",
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            "unstructured": "Zhao M.,Yang M., Ma H., Li X., Tan X., Li S., Yang Z., and Hoffman R. M., 2006. Targeted Therapy with a Salmonella typhimurium Leucine-Arginine Auxotroph Cures Orthotopic Human Breast Tumors in Nude Mice. Cancer Res. 66 (15), 7647- 7652"
        }
    ],
    "record": {
        "registrant": "Longman Publishers",
        "registered": "2026-09-08",
        "updated": "2026-09-20",
        "issue_number": 1,
        "source": "crossref-api",
        "source_agency": "Crossref (member 25296)"
    }
}

The Kernel Metadata Declaration

DOIMetadataKernel.xsd · namespace http://www.doi.org/2010/DOISchema · Attribute Value Sets 2.3 · draft — the agency's DOI name is filled in on accreditation

<?xml version="1.0" encoding="UTF-8"?>
<!-- DRAFT declaration: Smart Scholars is not yet a DOI Registration Agency. registrationAgencyDoiName is a marker (10.0/ is no RA's prefix) and is filled in on accreditation. -->
<kernelMetadata xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.doi.org/2010/DOISchema https://www.doi.org/doi_schemas/DOIMetadataKernel.xsd" xmlns="http://www.doi.org/2010/DOISchema">
  <referentDoiName>10.46243/jst.2021.v06.i04.pp32-38</referentDoiName>
  <primaryReferentType>Creation</primaryReferentType>
  <registrationAgencyDoiName>10.0/smart-scholars-draft</registrationAgencyDoiName>
  <issueDate>2026-10-04</issueDate>
  <issueNumber>1</issueNumber>
  <referentCreation>
    <name primaryLanguage="en">
      <value>Inhibition of Proliferation of K-562 Human Blood Cancer Cell Due To Opuntia Elatior Fruit Extract</value>
      <type>PrincipalTitle</type>
    </name>
    <identifier>
      <nonUriValue>10.46243/jst.2021.v06.i04.pp32-38</nonUriValue>
      <uri returnType="text/html" doesContentNegotiation="true">https://doi.org/10.46243/jst.2021.v06.i04.pp32-38</uri>
      <type>DOI</type>
    </identifier>
    <identifier>
      <uri returnType="text/html">https://www.jst.org.in/index.php/pub/article/view/433</uri>
      <type>URI</type>
    </identifier>
    <identifier>
      <uri>https://www.jst.org.in/index.php/pub/article/download/433/385</uri>
      <type>URI</type>
    </identifier>
    <identifier>
      <uri returnType="application/xml">https://www.jst.org.in/index.php/pub/article/download/433/2921</uri>
      <type>URI</type>
    </identifier>
    <identifier>
      <uri>https://www.jst.org.in/index.php/pub/article/view/433/385</uri>
      <type>URI</type>
    </identifier>
    <structuralType>Digital</structuralType>
    <mode>Visual</mode>
    <character>Language</character>
    <type>JournalArticle</type>
    <principalAgent>
      <name>
        <value>Chirag U. Narayankar</value>
        <type>PrincipalName</type>
      </name>
      <role>Author</role>
    </principalAgent>
    <principalAgent>
      <name>
        <value>M. Mane</value>
        <type>PrincipalName</type>
      </name>
      <role>Author</role>
    </principalAgent>
    <principalAgent>
      <name>
        <value>R. Patil</value>
        <type>PrincipalName</type>
      </name>
      <role>Author</role>
    </principalAgent>
    <principalAgent>
      <name>
        <value>Longman Publishers</value>
        <type>PrincipalName</type>
      </name>
      <role>Publisher</role>
    </principalAgent>
    <linkedCreation>
      <name>
        <value>Journal of Science &amp; Technology</value>
        <type>PrincipalTitle</type>
      </name>
      <identifier>
        <nonUriValue>2456-5660</nonUriValue>
        <type>ISSN</type>
      </identifier>
      <referentCreationRole>Part</referentCreationRole>
      <referentCreationSequenceIdentifier>
        <value>06</value>
        <type>VolumeNumber</type>
      </referentCreationSequenceIdentifier>
      <referentCreationSequenceIdentifier>
        <value>04</value>
        <type>IssueNumber</type>
      </referentCreationSequenceIdentifier>
      <referentCreationSequenceIdentifier>
        <value>32-38</value>
        <type>PageNumber</type>
      </referentCreationSequenceIdentifier>
    </linkedCreation>
    <language>en</language>
    <languageOfReferentContent>
      <language>en</language>
      <languageOfReferentContentType>Original</languageOfReferentContentType>
    </languageOfReferentContent>
    <creationDate>
      <date>2021-07-14</date>
      <creationDateType>PublicationDate</creationDateType>
    </creationDate>
  </referentCreation>
</kernelMetadata>
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